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Membranes were probed with anti-GCLC sc-390811 (200 ng/ml), anti-ACTB sc-69879 (5 ng/ml), and anti-Vinculin sc-73614 (200 ng/ml), both from Santa Cruz Biotechonology (Santa Cruz, CA, USA) followed by goat anti-mouse IgG conjugated to IR-800Dye (926-32210

a , b , The plasmids encoding full-length (FL) or truncated forms of OGT (M1 and M2) were introduced into HEK293T cells ( a )

Biotin: Important part of enzymes in the body that break down substances like fats, carbohydrates, and others

Samples were loaded into glass capillaries (Hirschmann ringcaps, 1 mm inner diameter) and sealed with Hemato-Seal capillary tube sealant (Fisherbrand)

Hepatology 40, 11701179

(Araliaceae), and its metabolite compound K(CK) have demonstrated the ability to enhance osteogenesis and angiogenesis by activating the Notch and Wnt/-catenin pathways in BMSCs and Human umbilical vein endothelial cells (HUVECs), respectively